Title:
SARS-CoV-2 neutralization and serology testing of COVID-19 convalescent plasma from donors with non-severe disease
Author:
Thomas J. Gniadek, Joshua M. Thiede, William E. Matchett, Abigail R. Gress, Kathryn A. Pape, Marc K. Jenkins, Vineet D Menachery, Ryan A. Langlois, Tyler D. Bold
Published:
bioRxiv, 10 August 2020 (preprint)
https://www.biorxiv.org/content/10.1101/2020.08.07.242271v1
Abstract:
We determined the antigen binding activity of convalescent plasma units from 47 individuals with a history of non-severe COVID-19 using three clinical diagnostic serology assays (Beckman, DiaSorin, and Roche) with different SARS-CoV-2 targets. We compared these results with functional neutralization activity using a fluorescent reporter strain of SARS-CoV-2 in a microwell assay. This revealed positive correlations of varying strength (Spearman r = 0.37-0.52) between binding and neutralization. Donors age 48-75 had the highest neutralization activity. Units in the highest tertile of binding activity for each assay were enriched (75-82%) for those with the highest levels of neutralization.