SARS-CoV-2 501Y.V2 escapes neutralization by South African COVID-19 donor plasma [Preprint - bioRxiv, 19 January 2021]

Title:

SARS-CoV-2 501Y.V2 escapes neutralization by South African COVID-19 donor plasma  

 

Authors:

Constantinos Kurt Wibmer, Frances Ayres, Tandile Hermanus, Mashudu Madzivhandila, Prudence Kgagudi, Bronwen E. Lambson, Marion Vermeulen, Karin van den Berg, Theresa Rossouw, Michael Boswell, Veronica Ueckermann, Susan Meiring, Anne von Gottberg, Cheryl Cohen, Lynn Morris, Jinal N. Bhiman & Penny L. Moore

 

Published:

bioRxiv, 19 January 2021

[This is a preprint.]

https://www.biorxiv.org/content/10.1101/2021.01.18.427166v1

Abstract:

SARS-CoV-2 501Y.V2, a novel lineage of the coronavirus causing COVID-19, contains multiple mutations within two immunodominant domains of the spike protein. Here we show that this lineage exhibits complete escape from three classes of therapeutically relevant monoclonal antibodies. Furthermore 501Y.V2 shows substantial or complete escape from neutralizing antibodies in COVID-19 convalescent plasma. These data highlight the prospect of reinfection with antigenically distinct variants and may foreshadow reduced efficacy of current spike-based vaccines.

bioRxiv, 10 August 2020 (preprint) - SARS-CoV-2 neutralization and serology testing of COVID-19 convalescent plasma from donors with non-severe disease

Title:
SARS-CoV-2 neutralization and serology testing of COVID-19 convalescent plasma from donors with non-severe disease

Author:
Thomas J. Gniadek, Joshua M. Thiede, William E. Matchett, Abigail R. Gress, Kathryn A. Pape, Marc K. Jenkins, Vineet D Menachery, Ryan A. Langlois, Tyler D. Bold

Published:
bioRxiv, 10 August 2020 (preprint)
https://www.biorxiv.org/content/10.1101/2020.08.07.242271v1

Abstract:
We determined the antigen binding activity of convalescent plasma units from 47 individuals with a history of non-severe COVID-19 using three clinical diagnostic serology assays (Beckman, DiaSorin, and Roche) with different SARS-CoV-2 targets. We compared these results with functional neutralization activity using a fluorescent reporter strain of SARS-CoV-2 in a microwell assay. This revealed positive correlations of varying strength (Spearman r = 0.37-0.52) between binding and neutralization. Donors age 48-75 had the highest neutralization activity. Units in the highest tertile of binding activity for each assay were enriched (75-82%) for those with the highest levels of neutralization.